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转录组学

降解组测序

背景简介


        microRNA(miRNA)是通过转录后水平调控的靶基因(mRNA)的表达来实现其重要生物学功能。在生物体内,miRNA除了抑制mRNA的翻译外,也会诱导mRNA被剪切降解,从而调控基因表达,发挥生物学功能。相比于动物,在植物中miRNA诱导基因剪切降解的方式会更为普遍。miRNA剪切靶基因会产生二个片段,5‘剪切片段和3’剪切片段,其中3‘剪切片段,包含有自由的5’单磷酸和3‘polyA尾巴,可被磁珠富集,并可在RNA连接酶的作用下连接接头,连接产物经扩增可用于下游的高通量测序。降解组测序正是对mRNA的降解片段进行测序,进而可靠地大规模实验鉴定miRNA靶基因的一种高效工具。




 
 


技术优势


用户文章数量最多:用户发表降解组文章约占全球降解组实验性文章总数1/3,国内最多。

业内领先的文库构建:样本起始量更低,建库步骤更少、测序读长更长,更真实反映样本降解组的丰度,显著提高数据准确性

数据分析软件优:使用自主开发的数据分析软件ACGT301-DEG101,并结合CleaveLand,分析可靠性经过数千个实验项目检验

项目经验丰富:国内最早开展降解组测序服务,成熟的研究组合方案,帮助用户研究成果快速发表。





 


 


技术路线


 



 


  

分析内容


 






样本类型


细胞,组织,总RNA等


建议总RNA起始量:50 μg,最低25 μg,浓度≥400 ng/μL


近期用户文章 

1. Li H, Peng T, Wang Q, Wu Y, Chang J, Zhang M, Tang G, Li C. (2017) Development of Incompletely Fused Carpels in Maize Ovary Revealed by miRNA, Target Gene and Phytohormone Analysis. Frontiers in Plant Science 8(1), 463.

2. Wang Q, Li T, Xu K, Zhang W, Wang X, Quan J, Jin W, Zhang M, Fan G, Wang M. (2016) The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete Pathogen Phytophthora sojae. Frontiers in Plant Science 7,

3. Tang F, Wei H, Zhao S, Wang L, Zheng H, Lu M. (2016) Identification of microRNAs Involved in Regeneration of the Secondary Vascular System in Populus tomentosa Carr. Frontiers in Plant Science 7(1),

4. Gao F, Wang N, Li H, Liu J, Fu C, Xiao Z, Wei C, Lu X, Feng J, Zhou Y. (2016) Identification of drought-responsive microRNAs and their targets in Ammopiptanthus mongolicus by using high-throughput sequencing. Scientific Reports 6(1), 34601.

5. Zhou R, Wang Q, Jiang F, Cao X, Sun M, Liu M, Wu Z. (2016) Identification of miRNAs and their targets in wild tomato at moderately and acutely elevated temperatures by high-throughput sequencing and degradome analysis. Scientific Reports 6(1), 33777.

6. Zhang J, Huang M, Liang J, Pan Y, Cheng L, Wu J, Tong Z. (2016) Genome-wide mining for microRNAs and their targets in Betula luminifera using high-throughput sequencing and degradome analyses. Tree Genetics & Genomes 12(5), 99.

7. Chen J, Zheng Y, Qin L, Wang Y, Chen L, He Y, Fei Z, Lu G. (2016) Identification of miRNAs and their targets through high-throughput sequencing and degradome analysis in male and female Asparagus officinalis. BMC Plant Biology 16(1), 1.

8. Yijun Meng, Dongliang Yu, Jie Xue, Jiangjie Lu, Shangguo Feng, Chenjia Shen& Huizhong Wang. (2016) A transcriptome-wide, organspecific regulatory map of Dendrobium officinale, an important traditional Chinese orchid herb. Scientific Reports.6:18864. doi: 10.1038/srep18864.

9. Marina Naoumkina, Gregory N. Thyssen, David D. Fang, Doug J. Hinchliffe, Christopher B. Florane and Johnie N. Jenkins. (2016) Small RNA sequencing and degradome analysis of developing fibers of short fiber mutants Ligon-lintles-1 (Li1) and −2 (Li2) revealed a role for miRNAs and their targets in cotton fiber elongation. BMC Genomics. 17:360. doi: 10.1186/s12864-016-2715-1.

10. Xu S, Liu N, Mao W, Hu Q, Wang G, Gong Y. (2016) Identification of chilling-responsive microRNAs and their targets in vegetable soybean (Glycine max L.). Scientific Reports.6:26619.doi: 10.1038/srep26619.

 

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