首页

首页

VariantBaits™ 液相捕获定制化Panel

定制产品

平台简介

VariantBaits™液相杂交捕获系统可以对客户感兴趣的基因组目标区域进行富集,并在Illumina、Ion Torrent和BGI等二代测序平台进行高通量测序。通过LC Sciences自主专利的μParaflo®微流体芯片大规模合成高质量寡核苷酸,从而制备超长RNA探针,对带有测序接头的基因组文库进行液相杂交,与目标区域序列互补配对的探针在杂交时特异性结合目的片段DNA,通过链霉亲和素磁珠与探针上的生物素标记结合,从而抓取并富集目的片段DNA。可用于从小到大规模的基因捕获,容忍某些局部模板的变异。

LC Sciences采用光原位合成原理,研发出以硅晶板为基础的大规模合成芯片平台μParaflo® 微流体芯片,是全球少数几家拥有此项技术的公司。通过微流控技术对核酸原料进行控制,经由控制光的封闭与否对合成的DNA反应进行控制,达到合成DNA的目的。相比于其他方式,此种DNA合成方法可以实现更大规模的合成以及更可控的DNA合成质量。在合成DNA时,当长度小于1000bp时,芯片合成成本远低于其他合成方式。在大规模合成DNA的领域当中,芯片合成占有绝对优势,也是主流选择。


技术优势

1 美国LC Sciences探针合成平台15年技术沉淀,30余篇论文支持
2 120nt超长RNA捕获探针覆瓦式覆盖目标区域,拥有更高的错配容忍度和捕获效率
3 独特的探针设计优化,充分利用探针与捕获区域的结合特性,减少冗余产生
4 可按客户需求个性化定制Panel,定制周期30个工作日


性能展示 





建库原理




定制流程  


  


订购信息



检测流程


 

合作成果

1.Gao X, Church, GM, et al. (2004) Accurate multiplex gene synthesis from programmable DNA chips. Nature 432, 1050-1054.

2.Porreca GJ, Zhang K, Li JB, Xie B, Austin D, Vassallo SL, LeProust EM, Peck BJ, Emig CJ, Dahl F, Gao Y, Church GM, Shendure J. (2007) Multiplex amplification of large sets of human exons. Nat Methods 4(11), 931-36.

3.Gnirke A, Melnikov A, Maguire J, et al. (2009) Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nat Biotechnol 27, 182–89.

4.Lira Mamanova, Alison J Coffey1, et al. (2010) Target-enrichment strategies for nextgeneration sequencing. Nat Methods 7(2):111-8.

5.Teer JK, Bonnycastle LL, et al. (2010) Systematic comparison of three genomic enrichment methods for massively parallel DNA sequencing. Genome Res 20(10), 1420-31.

6.Matzas M, Stähler PF, et al. (2010) High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing. Nat Biotechnol 28(12), 1291-94.

7.Nautiyal S, Carlton VE, Lu Y, Ireland JS, Flaucher D, Moorhead M, Gray JW, Spellman P, Mindrinos M, Berg P, Faham M. (2010) High-throughput method for analyzing methylation of CpGs in targeted genomic regions. Proc Natl Acad Sci 107(28), 12587-92.

8.Myllykangas S, Buenrostro JD, et al. (2011) Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing. Nat Biotechnol 29, 1024-27.

9.Diep D, Plongthongkum N, Gore A, Fung H, Shoemaker R, Zhang K. (2012) Library-free methylation sequencing with bisulfite padlock probes. Nature Methods 9(3), 270-2.

10.Labrie V, Buske OJ, Oh E, Jeremian R, Ptak C, Gasiūnas G, Maleckas A, Petereit R, Žvirbliene A, Adamonis K et al.(2016) Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging. Nature Structural &Molecular Biology 23(6):566-73.

11.Zaccai F, C T, Savitzki D, Zivony-Elboum Y, Vilboux T, Fitts EC, Shoval Y, Kalfon L, Samra N, Keren Z. (2017) Phospholipase A2-activating protein is associated with a novel form of leukoencephalopathy. Brain 140(2), 370-386.


 

杭州联川生物技术股份有限公司. All Rights Reserved.
浙ICP备15020535号